Main research lines

Annexin A6 and Signalling

AnxA6 is recruited to the plasma membrane after different stimuli due to a local increment of calcium levels. It has been shown that AnxA6 binds and targets numerous Ras/MAPK signalling pathway proteins such as p120GAP, Raf-1 and PKCα to the vicinity of the plasma membrane. There, AnxA6 works as a scaffolding protein that regulates these signalling pathways. In this project we study the molecular mechanisms that allow AnxA6 to regulate these signalling pathways and its implication in cell proliferation and cancer.

Continue reading >

Annexin A6 in Membrane Trafficking and Organization

AnxA6 is a highly dynamic protein involved in a number of cellular events linked to membrane transport such as the cholesterol delivery from late endosomes to the Golgi and the plasma membrane, the cholesterol-dependent recruitment of cPLA2 to the Golgi and its involvement in Golgi vesiculation, and the regulation of SNARE proteins. In this research line we study the molecular and cellular mechanisms that AnxA6 regulates in all these processes.

Continue reading >

Role of Annexin A6 During Liver Regeneration

Annexin A6 is an abundant protein in the liver. However, its depletion shows no phenotype in mice. Due to its relevant role of AnxA6 in lipid metabolism and cellular trafficking, we aim to study the bile synthesis and secretion in AnxA6 knock-out mice. At the same time, we will study the function of AnxA6 in a liver under metabolic and regenerative stress through 2/3 partial hepatectomy.

 

Continue reading >

Endosomes as signalling platforms

Besides K-Ras, Rac1 and Cdc42 are CaM binding proteins present in the endosomal compartment. Thus, we are analyzing the role of CaM in the EGFR signalling through H-Ras, K-Ras and Rac1 and the dynamics of actin in this location. Since another location and a very important place of action of Rac1, Cdc42, CaM and PKCδ is the plasma membrane, it is also essential to study the cellular processes activated by EGFR in plasma membrane in which proteins take part. Mainly, we will focus our investigations in endocytosis, mobility, formation of lamellipodia and cellular migration.

Continue reading >

Functional Regulation of Rac1 and Cdc42 by Calmodulin

On the basis of the preliminary results obtained recently and on the analysis of the aminoacidic sequence of these GTPases, it seems that CaM interacts with Rac1 and cdc42. Therefore, CaM through these small Rho GTPases could regulate cytoskeleton related cellular processes like cellular mobility and/or endocytic compartment functionality.

Continue reading >

Role of PKCδ in the degradation of EGFR and LDL. Relevance in different types of tumors

The PKCδ activity is necessary for the efficient degradation of the EGF-EGFR complex. The degradation of the EGF-EGFR depends on its own signaling, its ubiquitinylation and the subsequent traffic of the receptor to the multivesicular bodies (MBVs) /lysosomes. The role of PKCδ in the different processes that participate in the degradation of the receptor will be analyzed. In a comparative way, the role of PKCδ in the degradation of LDL will also be studied. LDL is transported to the early endosomes by binding to its specific receptor, but it does not participate in the later transport of LDL to lysosomes. In addition, our interest is focused on the correlation between the levels of PKCδ and EGFR in the putative abnormal traffic of the EGFR in different tumour lines.

Continue reading >

Caveolin and the intracellular trafficking of cholesterol, implications on cell metabolism, proliferation and apoptosis

Caveolins (CAVs) are essential components of caveolae; plasma membrane invaginations with reduced fluidity, reflecting cholesterol accumulation. CAV proteins bind cholesterol, and CAV’s ability to move between cellular compartments helps control intracellular cholesterol fluxes.

Continue reading >

Hepatic and Brain Mitochondrial Cholesterol in Health and Disease

Without CAV1, free cholesterol accumulates in mitochondrial membranes to promote membrane condensation, accumulation of reactive oxygen species and cell death. Thus mitochondrial cholesterol predisposes cells and animals to diseases such as steatohepatitis and neurodegeneration.

Continue reading >
Mechanisms of LD formation

Mechanisms of Lipid Droplet Biogenesis, Acumulation and Metabolism

Lipid droplets are ubiquitous organelles that collect, store and supply lipids in all mammalian cells and also in bacteria, yeast, fungi and plants. In a changing environment, mechanisms for efficient storage and timely delivery of lipids likely represented an important adaptive response for individual cells. Excessive or reduced accumulation of LDs is clearly undesirable, and is a cellular hallmark of prevalent human diseases such as hepatic steatosis and steatohepatitis, obesity, metabolic syndrome, diabetes, myopathies, lipodystrophies and arteriosclerosis but also have an important economical impact by production of food and biofuels.

Continue reading >

Our group in the news

Brief summary of the out-reach activities of the group

Continue reading >
Imagen de seguimiento