Membrane trafficking and Annexin A6 group

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Role of Annexin A6 During Liver Regeneration

Summary

Annexin A6 is an abundant protein in the liver. However, its depletion shows no phenotype in mice. Due to its relevant role of AnxA6 in lipid metabolism and cellular trafficking, we aim to study the bile synthesis and secretion in AnxA6 knock-out mice. At the same time, we will study the function of AnxA6 in a liver under metabolic and regenerative stress through 2/3 partial hepatectomy.

 

Description

Annexin A6 is one of the major proteins of the liver (0.25% of total protein). However, AnxA6-ko mice have normal phenotype, which keep its role in the hepatic physiology uncertain. To study the role of AnxA6 in the hepatic functioning we use the liver regeneration after partial hepatectomy in AnxA6-null mice. This process triggers the activation of the quiescent (remnant) hepatocytes into the cell cycle.

 

In this new project we aim to study the possible role of AnxA6 in the formation of lipid droplets and cholesterol metabolism and to determine the role of AnxA6 in the bile secretion.

Figura 4
Figure 4. Immunofluorescence staining of Adipofilin (green), actin cytoskeleton (red) and nuclei (blue).
Figure 5
Figure 5. Electron microscopy of liver section.
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